Blog - China FS Enzyme

Cost of Bromelain (Pineapple Proteinase)

September 12, 2023

120 yuan/kg. For specific inquiries, please consult customer service.

Cost of Papain (Papaya Proteinase)

September 12, 2023

Around 30-40 yuan/kg. For more details, please reach out to customer service.

Applications of Papain in Various Fields

September 12, 2023

Life Sciences Industry Healthcare Industry Papain supports the immune system in cancer therapy by controlling and altering white blood cells involved in immune responses. It reduces swelling and inflammation in joints and the prostate. Raw papaya-derived papain exhibits 90% activity. In 2012, Hitesch et al. studied the enzyme’s effects using agglutination formation and absorbance measurements. They identified enzyme-degrading compounds in the drug levetiracetam and granules with hazardous effects on cell systems. Spectrophotometric and thin-layer analyses of the drug showed favorable results. Papain also possesses antifungal properties. In 2010, Chukwuemka and Anthony reported that papaya could reduce fungal infections responsible for papaya decay. Their investigation of fungi from Rhizopus, Aspergillus, and Mucor demonstrated inhibition zones reducing mycelial growth, statistically showing that papaya could extend shelf life. Papain reduces allergic reactions in leaky gut syndrome by decreasing hydrochloric acid and lowering gelatin intolerance. It has been shown to offer significant pain relief and anti-inflammatory effects in head and tooth pain allergies without side effects. Papain plays a vital role in skin and wound care. In Hawaii and Tahiti, concentrated papain extracts from...

What is Papain?

September 12, 2023

Papain is an enzyme with proteolytic functions, classified as a cysteine protease. It belongs to a superfamily and consists of a single polypeptide chain containing three disulfide bonds and a thiol group essential for its enzymatic activity. Papain is derived from green papaya (see Figure 1), and its activity depends on the ripeness of the fruit. This enzyme plays a crucial role in various biological functions, such as hydrolyzing short peptides, proteins, esterified amino acids, and amide bonds, making it useful in both medical and food applications. It specifically cleaves positively charged amino acid peptide chains, mainly targeting lysine, arginine, and phenylalanine residues. Molecular Weight: 23,406 DaOptimal pH for Activity: 6.0-7.0Optimal Temperature for Activity: 65 °CIsoelectric Point (pI): 8.75 to 9.55Spectral Properties: λmax: 278 nmExtinction Coefficient: E1% = 25, EmM = 57.6 (280 nm) Inhibitors: E-64, cysteine dihydrochloride, trypsin inhibitors, ibuprofen, and leupeptin trifluoroacetate. Unit Definition: Under conditions of 25 °C and pH 6.2, one unit hydrolyzes 1.0 µmole of N-α-benzoyl-L-arginine ethyl ester per minute. Source: Isolated from green papaya. Specificity Papain can digest a wide range of protein substrates,...

Benefits of Papain

September 12, 2023

Papain aids in digestion and improves appetite. It’s a type of proteolytic enzyme containing cysteine, commonly used in the brewing and food industries. Papain can enhance the digestion and absorption in the gastrointestinal tract by breaking down fats into fatty acids, gradually increasing the nutritional value of food, making it easier for patients to digest. It is known for its benefits in strengthening the spleen and stomach, relieving constipation, and alleviating stomach discomfort. Papain is a low-specificity proteolytic enzyme found in papaya roots, stems, and fruit, with higher concentrations in unripe papaya. It has significant benefits for patients, and consuming more of it can be quite helpful.

What are the Uses of Papain?

September 12, 2023

Papain aids in protein digestion and can be used for conditions like chronic indigestion and gastritis. It's also effective for preventing adhesions during abdominal surgery; animal studies show it outperforms trypsin in preventing adhesion recurrence. The juice from unripe papaya can digest damaged tissue in anthrax lesions without affecting healthy tissue, although the effect is less pronounced in ripe fruit. Papain solutions can dissolve small blood clots, especially when combined with trace amounts of glutathione. It can also break down thick pus, although antibiotics like tetracycline, chloramphenicol, and streptomycin can slow this process, while penicillin and sulfa drugs do not affect it.

Properties of Papain

September 12, 2023

Papain is a mixture of proteases refined from the papaya plant (Carica papaya) and appears as a white to light brown amorphous powder or granules. It is slightly hygroscopic and has a characteristic smell of hydrogen sulfide. It is somewhat soluble in water and glycerin, but insoluble in ether, ethanol, and chloroform. The aqueous solution is colorless to light yellow and can sometimes appear milky. Its optimal pH ranges from 5.0 to 8.0, with an ideal temperature of 65 °C; it is sensitive to denaturation. Papain contains several proteases, including papain itself, papaya latex protease, and proteolytic enzymes. Papain is a thiol protease with relatively broad specificity, capable of breaking down more types of protein than pancreatic protease. It consists of a single polypeptide chain made up of 211 amino acid residues, with a molecular weight of 23,000 and an isoelectric point (pI) of 9.6. Activators include cysteine, sulfides, bisulfites, and EDTA, while inhibitors include thiol reagents and hydrogen peroxide. Papain comprises about 10% of soluble proteins; papaya latex protease makes up approximately 45% with a molecular weight of 36,000,...

The Hyped-Up Food Additive: Why Are Indians Obsessed with Papain?

September 12, 2023

Have you ever come across a curious news story about how Indians love growing papayas and, even when the fruit is still unripe, they make cuts all over it? In just seconds, a milky white liquid oozes from the papaya, and the locals quickly collect it in containers to sell. In some parts of India, people even make a living off of this papaya sap. So, why do people slice into the unripe papaya to gather this white liquid? What makes it so valuable? It turns out that the process of cutting into an unripe papaya and collecting the milky sap is highly specialized. This variety of papaya is not the edible kind—its nutritional value is low, but its economic value is high. The fruit doesn’t need to fully ripen. Once it grows large enough, people cut into its surface, and the papaya releases that milky substance, known as papain. Papain is a proteolytic enzyme, meaning it breaks down proteins, and it has protease activity. Papain isn’t just popular in Southeast Asia—it has a similarly elevated status in China, where...

Ingredients to Know: Papain

September 12, 2023

If you want effective skincare, having a solid understanding of ingredients is crucial. It’s not just about product marketing; it’s about the actual components. Today, let’s talk about “papain” in skincare products. What is Papain? Papain, also known as papaya enzyme, is a proteolytic enzyme found in the roots, stems, leaves, and fruit of the papaya plant. With its high cysteine content, it boasts strong activity and good stability, making it a natural and safe option for removing dead skin cells from the skin’s surface. Benefits of Papain Who Can Use Papain? Due to its metabolic effects on keratin, those with sensitive skin should use it cautiously. As a supportive ingredient for whitening, it’s suitable for healthy skin, but it’s important to ensure proper hydration while using it.

Papain: Baking, Meat Tenderizing, Wine Clarifying, Soy Sauce Brewing… The Food Industry Loses Quality Without It

September 12, 2023

Because of my profession, I have many friends in the food industry. After years of observation, I’ve noticed that any veteran in food R&D or procurement is very familiar with one product—papain. To put it a bit dramatically, papain is to food what vitamins are to the human body; without it, the freshness, flavor, and texture of food drops a grade. In food manufacturing, it not only reduces or replaces harmful chemical additives, making products greener and healthier, but also lowers production costs and increases yield—a true win-win-win! Thanks to its outstanding qualities, papain is now widely used across various sectors of the food industry, including baking, meat tenderizing, wine clarifying, soy sauce brewing, seasonings, protein powders, and animal and plant protein processing and hydrolysis. So, what exactly does papain do in these sectors? Let’s dive into the practical applications of papain. 01 Papain in the Baking Industry Papain can reduce the gluten strength in flour/dough, improving its plasticity and physicochemical properties by hydrolyzing large protein molecules into short peptides and amino acids. Lastly, it can correct the flavor impact...

Benefits of Papain

September 12, 2023

Papain is an active enzyme found in papaya that offers several health benefits. It can help boost the immune system, reduce inflammation and pain, and aid in digestion. Additionally, it provides relief from itching and pain caused by insect bites and can promote the healing of wounds. Papain may also be effective in preventing scars and improving their treatment. While it’s beneficial to use papain for nutritional and health support, it should not be considered a substitute for medication. It’s important not to rely solely on its therapeutic effects or use it indiscriminately.

Uses of Papain

September 12, 2023

Animal Feed IndustryPapain is used as a feed additive to enhance protein sources, improve absorption, and increase feed efficiency, ultimately reducing costs. It's beneficial for livestock digestion and promotes growth, making it suitable for use in feeds for pigs, cows, sheep, chickens, ducks, geese, fish, and shrimp. It can also be added to high-grade composite fertilizers for fruits and vegetables. Pharmaceutical IndustryMedicines containing papain can help treat cancer, tumors, lymphocytic leukemia, various pathogens, and tuberculosis. They provide anti-inflammatory effects, support bile production, relieve pain, and assist digestion, making them useful for treating gynecological issues, glaucoma, bone hyperplasia, wound healing, blood type identification, and insect bites. Food IndustryPapain facilitates enzymatic reactions that hydrolyze large protein molecules into smaller peptides or amino acids. It's used in the hydrolysis of animal and plant proteins to create tenderizing powders, hydrolyzed fetal serum, hydrolyzed soy, cookie softeners, noodle stabilizers, beer clarifiers, high-end oral liquids, health foods, soy sauce brewing, and fermentation agents. This effectively enhances protein utilization, significantly boosts nutritional value, and lowers costs, benefiting digestion and absorption. Beauty and CosmeticsWhen added to cosmetics containing...

Benefits and Effects of Bromelain

September 12, 2023

Bromelain is an anti-edema and anti-inflammatory agent. When taken orally, it enhances the hydrolysis of fibrin in the body, helping to dissolve fibrin or blood clots that are obstructing tissues. This improves local circulation of bodily fluids and aids in reducing inflammation and swelling. Additionally, when used alongside antibiotics or chemotherapy drugs, bromelain can accelerate the penetration and diffusion of these medications to the affected areas.

The Amazing Bromelain

September 12, 2023

Bromelain is a natural plant enzyme extracted from the fruit, leaves, and stem of pineapples. It is widely used in both the food and pharmaceutical industries. Bromelain is a glycoprotein composed of various enzymes with different molecular weights and structures, including at least five types of proteolytic enzymes. In addition, it may contain phospholipases, peroxidases, cellulases, other glycosidases, and non-protein substances. It is more effective than the body's own proteases at breaking down food proteins in the digestive tract and can increase the permeability of the intestinal mucosa, aiding in the absorption and utilization of amino acids. Bromelain has many medical applications. Its first use in medicine was in 1957, when it was successfully applied to inhibit certain inflammatory metabolites that would otherwise accelerate and worsen inflammatory responses. Bromelain is an anti-inflammatory agent without the side effects typically associated with drugs. It is used for sports injuries, trauma, arthritis, and other types of swelling. Its primary applications include treatment for sports injuries, gastrointestinal issues, phlebitis, sinusitis, and post-surgical recovery. A dosage of 200 mg of bromelain has been shown to...

Bromelain Can Help Relieve Inflammation

September 12, 2023

Pineapple is one of the most beloved tropical fruits worldwide. However, if you frequently browse the internet, you may have come across articles claiming that pineapple is “eating you.” What sparked such statements? It's bromelain. Beyond this notable fact, bromelain has garnered attention in the medical community for its impressive health benefits. If you're curious about why bromelain is gaining popularity, keep reading to learn more about this pineapple-derived compound. What is Bromelain? Bromelain, or pineapple extract, is a compound made up of proteolytic enzymes typically found in pineapples. While it's well-known for aiding digestion and breaking down proteins, its functions extend far beyond that. The good news is that bromelain is available as a supplement and can be used as an adjunct treatment for various conditions. The history of bromelain is closely tied to pineapple, as it is the only known major source of these enzymes. Chemist Vicente Marcano first discovered bromelain in 1891, which was later isolated by another chemist, Russell Henry Chittenden. Initially referred to as "bromelin," it was described as "a proteolytic enzyme in pineapple juice."...

Bromelain in Supplement Research and Use: How Should It Be Taken?

September 12, 2023

When it comes to pineapples, Mother Nature really nailed it. All kinds of plants, fruits, and vegetables carry nourishing and health-promoting properties that can make you feel better physically and mentally. But this spiky, fragrant, and sweet tropical fruit packs a particularly powerful punch, partly because it contains a substance called bromelain. This enzyme benefits your body from head to toe, from respiratory health to digestive support. So, adding some pineapple to your morning smoothie or enjoying a slice is a great idea. However, you should understand the ins and outs of this powerful enzyme extract, as well as the best ways to take bromelain supplements. What Is Bromelain? Bromelain—also known as pineapple enzyme or pineapple extract (Latin name Ananas comosus)—is a group of enzymes found in the fruit and stems of the pineapple plant. While it originated in the Americas, you can find it growing in tropical and subtropical climates around the world. Historically, indigenous peoples of Central and South America used pineapples to address various health needs, including digestion (like occasional bloating) and promoting healthy inflammatory responses. So...

Is Bromelain an Antibiotic?

September 12, 2023

No! Bromelain, also known as pineapple enzyme or pineapple protease, is a natural plant enzyme extracted from the stem, leaves, and skin of pineapples. It undergoes purification, concentration, enzyme immobilization, and freeze-drying to obtain a fine gray powder with a molecular weight of 33,000 and an isoelectric point of 9.55. Higher quality bromelain is derived from the pineapple’s core, processed through ultrafiltration, and freeze-dried. It is widely used in the food and pharmaceutical industries. Applications of Bromelain in Medicine and Supplements Antibiotics are secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher plants and animals, which possess the ability to interfere with the development of other living cells. The distinction is clear and requires no further elaboration. If you have any questions, feel free to ask!

Source and Uses of Bromelain

September 12, 2023

Bromelain is a food additive primarily extracted from the stems and leaves of pineapples. Its main uses include: Bromelain from Guangxi is particularly renowned, as this region is known for its high-quality pineapples.

Why does eating pineapple make your mouth tingle? What is bromelain, and what does it do?

September 12, 2023

Are you afraid of fruit? Some people might find it strange: fruit is sweet and tangy, so what’s there to fear? For me, the fruit I fear the most is pineapple. This is because when I was a kid, eating pineapple always left my mouth feeling prickly and numb; once, it even made me bleed… This experience left a significant psychological mark on my young mind. As I grew up, I learned that this was all due to a substance in pineapples—bromelain. This "painful" little guy showed up on health supplement shelves one day?! What’s going on here? What is Bromelain? Pineapple is a fruit native to South America and is an important part of traditional Hawaiian medicine. It’s one of the richest sources of bromelain in the world. Bromelain consists of several endopeptidases and compounds, such as phosphatases, glucosidases, peroxidases, cellulases, carboxypeptidases, and protease inhibitors. Our star today—bromelain—isn’t extracted from the flesh of the pineapple due to its low content but from the stem or core of the pineapple. In folk medicine, pineapple is widely used to treat various...

Effects and Side Effects of Bromelain

September 12, 2023

Bromelain is a type of dietary supplement found naturally in pineapples, but there are also specific bromelain supplements extracted from the fruit to make consumption easier. While many people are familiar with pineapples, they often don’t know much about bromelain, so let’s discuss its effects. Effects of Bromelain Though bromelain is classified as a nutritional supplement, many people are unaware of its benefits. Bromelain helps the body absorb antibiotics and can reduce gastrointestinal side effects. However, for those allergic to antibiotics, consuming pineapple can worsen allergic reactions. Bromelain is absorbed quickly by the body and is relatively inexpensive, making it suitable for people with nutritional deficiencies. Bromelain (similar to papain found in papayas) interferes with enzyme systems and effectively protects the digestive tract from stomach acid. Side Effects of Bromelain While bromelain has several benefits, many are unaware of its potential side effects. As a natural digestive enzyme, it can aid digestion and relieve intestinal burdens. However, individuals with gastrointestinal inflammation should avoid it, as it may exacerbate their condition. Additionally, those with nausea, heartburn, or gastroesophageal reflux can benefit...